Eisa Mahyari's GitHub Page
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Management | Statistics & Computer Science | Biology & Medicine |
---|---|---|
- Project coordination | - Machine Learning & AI | - Single-cell & spatial OMICs |
- Operational & cross-functional leadership | - Fine-tuning of LLM on specialized domains | - Translational research |
- Multi-disciplinary collaboration | - Software engineering | - Reproduction & infertility |
- Data-driven strategic planning & consultation | - Statistical & mathematical modeling | - Immunology, autoimmunity, & Gender-bias of immune function |
- Budgeting & resource allocation | - Biostatistics | - Hormonal regulation of immune function |
- Stakeholder communication | - Cloud computing (Slurm, AWS, Google) | - Infectious diseases HIV, MTb, Yellow Fever, Dengue, Zika, CMV, and SARS-CoV-2 |
- Grant writing | - Translational & clinical trial data analytics | - PBPK modeling & pharmacokinetics |
I am a computational biologist driven by applied machine learning and AI for translational biomedical research. I am an expert at statistical modeling and advanced data analysis. In medicine, I specialize in immunology, infectious diseases, and reproductive biology as a biologist. I enjoy leading diverse projects, coding, performing statistical analyses, and converting complex biological data into actionable insights alongside principal investigators and cross-disciplinary teams. I am most valued as an innovative and statistical thinker and methods, and my communication skills in collaborations with internal and external stakeholders.
As R. Assistant Professor at OHSU/ONPRC, I deploy computational pipelines, develop interactive data visualization platforms, secure and manage research funding through competitive grants, and lead the strategic development of AI/ML solutions for research. I also develop statistically based plans, projections, SOPs to manage internal and external functions.
My research in this role focuses on integrating high-dimensional multi-omics datasets, performing cross-species analysis, and employing statistical inference and computational omics to support clinical and preclinical initiatives. Furthermore, I actively consult across labs, providing technical training and mentorship to graduate students and guiding their research and career development.
My career has been defined by consistent advancement and a focus on statistics, machine learning, and advanced data analytics relevant to translational and clinical research. I have played a pivotal role in designing, implementing, and analyzing extensive research projects, combining wet lab and in silico methodologies to develop hypotheses and transform data into actionable outcomes. My work upholds rigorous quality control across various omics data types to ensure integrity and reproducibility. Through statistical consultations and analyses, I have contributed significantly to clinical study documentation, scientific publications, presentations, grants, and regulatory filings in academic and industry settings.
Currently, I am interested in leading and developing teams to create software, pipelines, and testing methodologies tailored for analyzing and translating complex biomedical data, supporting impactful translational research by integrating data types, and applying robust statistical methodologies and computational tools. With the advent of large language models (LLMs), such tasks and aims can be rapidly scaled; thus, my research includes fine-tuning, testing, and deploying specialized LLM for biomedical research.
Computation Group Lead, Research Assistant Professor
Lab of Jacob D. Estes, Ph.D. - Vaccine & Gene Therapy Institute (VGTI), ONPRC-OHSU
Research Assistant Professor, Computational, OMICs, Translational Mammalian Reproduction
Lab of Donald F. Conrad, Ph.D. - Division of Genetics, ONPRC-OHSU
Computational Biologist, Molecular Immunopathology - Research Associate
Lab of Benjamin N. Bimber, Ph.D. - Primate Genetics, ONPRC-OHSU
Biostatistics & Computational Biology Consultant
Drug Development & Testing for Nonalcoholic Steatohepatitis (NASH) - CytoDyn, USA
Computational Biologist, Reproductive Biology - Research Associate
Lab of Donald F. Conrad, Ph.D. - Reproductive Genetics, ONPRC-OHSU
2016 - 2018 Doctoral Candidate, DMICE-BCB, OHSU. Developed novel machine learning framework for automated classification of rare cells in single-cell RNA-seq data. 2013 - 2016 Doctoral Student, DMICE-BCB, OHSU.
Research Involvement:
Genome-based Bioinformatics, Assessment of bioinformatics pipelines identifying genomic structural variants across several sequenced Gibbons. Lucia Carbone Ph.D.
Computational phenotyping in cancer cell lines, CyTOF-based computational analysis of phenotypic heterogeneity in breast cancer-derived cell in the context of combinatory anti-cancer drug treatments to identify sensitive phenotypic subtypes within several breast cancer cell lines. Paul T. Spellman Ph.D.
Computational immunophenotyping, CyTOF-based computational immunophenotypic analysis of a unique population of tuberculosis (TB)-recognizing T-cells known as mucosal-associated invariant T (MAIT) cells. David M. Lewinsohn M.D./Ph.D.
2008 - 2012 Sr. Research Associate, Louis Picker M.D., Vaccine & Gene Therapy Institute (VGTI), OHSU. Contribution: Performed experiments, analyzed flow cytometric data, performed statistical tests and developed reports and presentation materials.
2005 - 2007 Research Associate, Clinical Research Investigative Studies Program (CRISP) and Critical Care Academics Associates Program (CCAAP), OHSU. Contribution: Acquired clinical data from patient charts for ongoing studies and identified candidates for enrollment into qualifying research in the emergency department and the intensive care units.
Post-Ph.D. – Mammalian Reproduction, Oregon Health & Science University (OHSU), Oregon National Primate Research Center (ONPRC), Division of Genetics
Advisor: Prof. Don F. Conrad
Ph.D. – Bioinformatics and Computational Biology, OHSU, Department of Medical Informatics and Clinical Epidemiology (DMICE), Division of Bioinformatics and Computational Biology (BCB), Portland, OR
Dissertation: “Robust and reproducible classification of rare cellular subsets/signatures (RCS) in single-cell technologies within a transfer learning framework.” (doi:10.6083/NX9EZJ)
Committee: Shannon K. McWeeney, Ph.D., David M. Lewinsohn, M.D./Ph.D., Michael Mooney, Ph.D., Christina Zheng, Ph.D., Evan Lind, Ph.D.
(2013 – 2018)
M.S. – Biochemistry and Molecular Biology, OHSU, Division of Environmental and Biomolecular Systems, Portland, OR
Thesis: “A six-compartment Physiologically Based Pharmacokinetic (PBPK) Markov-chain Monte Carlo Model to simulate and quantify nicotine metabolism in human populations.”
(2006 – 2008)
B.S. – Biology and Chemistry, Portland State University (PSU), Portland, OR
(2002 – 2006)
“The Human Infertility Single-cell Testis Atlas (HISTA)”: A web-based single-cell atlas that enables exploration and hypothesis testing of testis-specific cell types and genomic mechanisms in the context of infertility.
“Spatial-Omics Testis Atlas (SPATA) Suite”: A suite of tools to explore testis spatial and single-cell transcriptomic data across multiple species, facilitating comparative analysis and cross-species hypothesis generation in testis biology.
“Mouse Spatial-Omics Testis Atlas (MoSpaTA)”: Includes spatial data from various technologies like StereoSeq, Curio, and 10X Genomics to provide a comprehensive view of mouse testis at different developmental stages.
“Mouse Developmental Single-cell Testis Atlas (MoDSTA)”: A curated atlas of mouse testis development from embryonic stages to adulthood, supporting developmental and reproductive research.
“Rhesus macaque Spatial-Omics Testis Atlas (RheSpaTA)”: A platform housing spatial data from rhesus testis, enabling detailed investigation into non-human primate testis biology.
“homo Sapian Spatial-Omics Testis Atlas (SapSpaTA)”: A spatial-omics dataset for human testis, offering insights into gene expression patterns and cell type-specific information related to human reproductive health.
“ShinySDA R Package”: An R-based Shiny application for processing, browsing, and visualizing Sparse Decomposition of Arrays (SDA) objects, allowing real-time analysis of high-dimensional data.
“Rhesus Immunome Reference Atlas (RIRA) R Package”: A multi-tissue single-cell RNA-seq atlas from rhesus macaque immune cells, complete with pipelines for cell type annotation and data integration for cross-species research.
“Single-cell Custom Functions (scCustFx) R Package”: A library of analytical and visualization functions for single-cell and spatial omics analysis, supporting custom workflows in R.
“CellMembrane R Package”: A toolkit of high-level wrappers and visualization tools for single-cell omics data processing and analysis designed for fault-tolerant pipelines and enhanced QC visualizations.
“TCR Enrichment Analysis (TEA)”: A web-based visualization interface to evaluate differential enrichment of tissue-specific TCR clonotype.
Mahyari, Eisa, et al. “Rhesus Immune Reference Atlas (RIRA)” Accepted Cell Genomics (2025).
Mahyari, Eisa, et al. “Divergent B-cell and cytotoxic TNK cell activation signatures in HLA-B27-associated ankylosing spondylitis and acute anterior uveitis” Frontiers (2025). link
Mariya B. Shapiro, Tracy Ordonez, Shilpi Pandey, Eisa Mahyari, Kosiso Onwuzu, Jason Reed, Heather Sidener et al. “Immune perturbation following SHIV infection is greater in newborn macaques than in infants.” JCI insight (2024).
Sujittra Khampang, Chanchao Lorthongpanich, Chuti Laowtammathron, Phatchanat Klaihmon, Sukanya Meesa, Wichuda Suksomboon, Nittaya Jiamvoraphong, Eisa Mahyari et al. “The dynamic expression of YAP is essential for the development of male germ cells derived from human embryonic stem cells.” Scientific Reports 14, no. 1 (2024): 15732.
Lillepea, Kristiina, Anna-Grete Juchnewitsch, Laura Kasak, Anu Valkna, Avirup Dutta, Kristjan Pomm, Olev Poolamets, Eisa Mahyari et al. “Toward clinical exomes in diagnostics and management of male infertility.” The American Journal of Human Genetics 111, no. 5 (2024): 877-895.
Juchnewitsch, Anna-Grete, Kristjan Pomm, Avirup Dutta, Erik Tamp, Anu Valkna, Kristiina Lillepea, Eisa Mahyari et al. “Undiagnosed RASopathies in infertile men.” Frontiers in endocrinology 15 (2024): 1312357.
Mahyari, Eisa, Katinka A. Vigh‐Conrad, Clément Daube, Ana C. Lima, Jingtao Guo, Douglas T. Carrell, James M. Hotaling, Kenneth I. Aston, and Donald F. Conrad. “The human infertility single‐cell testis atlas (HISTA): an interactive molecular scRNA‐Seq reference of the human testis.” Andrology (2024).
Liina Nagirnaja, Alexandra M. Lopes, Wu-Lin Charng, Brian Miller, Rytis Stakaitis, Ieva Golubickaite, Alexandra Stendahl, Tianpengcheng Luan, Corinna Friedrich, Eisa Mahyari, […], Kristian Almstrup, Kenneth I Aston, Donald F Conrad “Diverse monogenic subforms of human spermatogenic failure”, Nature Communications, 13, 1, (2022). link
Yoshinori Fukazawa, Jeremy Smedley, Bryan Randall, Eisa Mahyari, Carly Starke, Richard Lum, Manuel Medina, Benjamin Varco-Merth, Kathleen Busman-Sahay, Miranda Fisher, “Mesenteric lymph nodes are sites of increased SIV RNA expression in SIV-infected rhesus macaques on antiretroviral therapy”, Journal of Medical Primatology, 51, 5, 320-320, (2022)
GW McElfresh, Gregory Boggy, Eisa Mahyari, Michael Stutz, Abigail Ventura, Scott Hansen, Louis Picker, Benjamin Bimber, “Single cell transcriptomic profiling of early tuberculosis infection and granuloma formation in rhesus macaque”, Journal of Medical Primatology, 51, 5, 313-314, (2022)
Eisa Mahyari, Abigail Ventura, Eric McDonald, Gregory Boggy, GW McElfresh, Scott Hansen, Louis Picker, Benjamin Bimber, “Rhesus Immunome Reference Atlas (RIRA): A multi-tissue single-cell landscape of immune cells”, Journal of Medical Primatology, 51, 5, 296-296, (2022)
Gregory J Boggy, GW McElfresh, Eisa Mahyari, Abigail B Ventura, Scott G Hansen, Louis J Picker, Benjamin N Bimber “BFF and cellhashR: analysis tools for accurate demultiplexing of cell hashing data.” Bioinformatics (2022). link
Eisa Mahyari, Jingtao Guo, Ana C. Lima, Daniel P. Lewinsohn, Alexandra M. Stendahl, Katinka A. Vigh-Conrad, Xichen Nie, Liina Nagirnaja, Nicole B. Rockweiler, Douglas T.Carrell, James M.Hotaling, Kenneth I.Aston, Donald F.Conrad “Comparative single-cell analysis of biopsies clarifies pathogenic mechanisms in Klinefelter syndrome.” The American Journal of Human Genetics 108, no. 10 (2021): 1924-1945. link
N Haigwood, M Shapiro, T Cheever, S Pandey, E Mahyari, K Onwuzu, J Reed, H Sidener, J Smedley, L Colgin, A Lewis, A Johnson, B Bimber, J Sacha, A Hessell “Immune perturbation is more profound in newborn than in infant macaques during acute SHIV infection.” Journal of the International AIDS Society (2021). link
Bruce K. Patterson, Harish Seethamraju, Kush Dhody, Michael J. Corley, Kazem Kazempour, Jay Lalezari, Alina PS Pang, Eisa Mahyari et al. “CCR5 inhibition in critical COVID-19 patients decreases inflammatory cytokines, increases CD8 T-cells, and decreases SARS-CoV2 RNA in plasma by day 14.” International Journal of Infectious Diseases 103 (2021): 25-32.Link
Husam Taher, Eisa Mahyari, Craig Kreklywich, Luke S Uebelhoer, Matthew R McArdle, Matilda J Moström, Amruta Bhusari, Michael Nekorchuk, Travis Whitmer, Elizabeth A Scheef, Lesli M Sprehe, Dawn Roberts, Colette M Hughes, Kerianne A Jackson, Andrea N Selseth, Abigail B Ventura, Yujuan Yue, Kimberli A Schmidt, Jason Shao, Paul T Edlefsen, Jeremy Smedley, Richard J Stanton, Michael K Axthelm, Jacob D Estes, Scott G Hansen, Amitinder Kaur, Peter A Barry, Benjamin N Bimber, Louis J Picker, Daniel N Streblow, Klaus Früh, Daniel Malouli. “In vitro and in vivo characterization of a recombinant rhesus cytomegalovirus containing a complete genome.” PLoS pathogens, 2020. Link.
Helen L Wu, Whitney C Weber, Christine Shriver‐Munsch, Tonya Swanson, Mina Northrup, Heidi Price, Kimberly Armantrout, Mitchell Robertson‐LeVay, Jason S Reed, Katherine B Bateman, Eisa Mahyari, Archana Thomas, Stephanie L Junell, Theodore R Hobbs, Lauren D Martin, Rhonda MacAllister, Benjamin N Bimber, Mark K Slifka, Alfred W Legasse, Cassandra Moats, Michael K Axthelm, Jeremy Smedley, Anne D Lewis, Lois Colgin, Gabrielle Meyers, Richard T Maziarz, Benjamin J Burwitz, Jeffrey J Stanton, Jonah B Sacha. “Viral opportunistic infections in Mauritian cynomolgus macaques undergoing allogeneic stem cell transplantation mirror human transplant infectious disease complications.” Xenotransplantation, 2020. Link.
Emily B Wong, Marielle C Gold, Erin W Meermeier, Bongiwe Z Xulu, Sharon Khuzwayo, Zuri A Sullivan, Eisa Mahyari, Zoe Rogers, Hénrik Kløverpris, Prabhat K Sharma, Aneta H Worley, Umesh Lalloo, Prinita Baijnath, Anish Ambaram, Leon Naidoo, Moosa Suleman, Rajhmun Madansein, James E McLaren, Kristin Ladell, Kelly L Miners, David A Price, Samuel M Behar, Morten Nielsen, Victoria O Kasprowicz, Alasdair Leslie, William R Bishai, Thumbi Ndung’u, David M Lewinsohn. “TRAV1-2+ CD8+ T-cells including oligoconal expansions of MAIT cells are enriched in the airways in human tuberculosis.” Nature Communications biology, 2019. NCBI link.
Yoshinori Fukazawa, Haesun Park, Mark J. Cameron, Francois Lefebvre, Richard Lum, Noel Coombes, Eisa Mahyari, Shoko I. Hagen, Jin Young Bae, Marcelo Delos Reyes, Tonya Swanson, Alfred W. Legasse, Andrew Sylwester, Scott G. Hansen, Andrew T. Smith, Petra Stafova, Rebecca Shoemaker, Yuan Li, Kelli Oswald, Michael K. Axthelm, Adrian McDermott, Guido Ferrari, David C. Montefiori, Paul T. Edlefsen, Michael Piatak, Jeffrey D. Lifson, Rafick P. Sékaly, and Louis J. Picker. “Lymph node T cell responses predict the efficacy of live attenuated SIV vaccines.” Nature Medicine, 2012. NCBI link.